Professor Federico Calegari
Professor Calegari got his master degree at Università Statale di Milano, Milan, Italy, course in Biology. After that he did his PhD in the Laboratory of Dr. Patrizia Rosa, CNR-Center of Cellular Pharmacology.
From 2015 he is a Professor for Proliferation of Mammalian Neural Stem Cells and is the leader of his research team at CRTD – Center for Regenerative Therapies Dresden. The field of their work is focused on Neurogenesis and development of neurons from Neural Stem Cells (NSC).
The goal of his teams is to understand the mechanisms underlying the expansion of NSC in order to control the generation of neurons in the mammalian brain. Controlling neurogenesis during development or adulthood is important not only to understand brain development and the role of adult neurogenesis in cognitive function but also to use NSC for brain recovery during aging, neurodegenerative disease or injury. How they did it?
Prof. Calegari and his team found that the duration of G1 phase of the cell cycle plays a key role in the genesis of neural cells. They describe a system that allows the control of neural stem/progenitor cell (altogether referred to as NSC) expansion in the mouse embryonic cortex or the adult hippocampus by manipulating the expression of the cdk4/cyclinD1 complex, a major regulator of the G1 phase of the cell cycle and somatic stem cell differentiation. The team used two approaches for overexpression of the regulating complex in the NSC – the first one is via electroporation and highly concentrated HIV-derived viruses injected into the gyrus dentatus of the adult mouse hippocampus.
In particular, by manipulating the expression of the cdk4/cyclinD1 complex, their system allows the temporal control of NSC expansion and their switch to differentiation, thus, ultimately increasing the number of neurons generated in the mammalian brain. This approach is important for basic research and using somatic stem cells for therapy of the mammalian central nervous system while providing a better understanding of i) stem cell contribution to tissue formation during development, ii) tissue homeostasis during adulthood, iii) the role of adult neurogenesis in cognitive functions, and perhaps, iv) better using somatic stem cells in models of neurodegenerative diseases.
ICMS 2019 is bringing you a lecture that should not be missed by one of the most highly respected Neuroscientists in the world!